Police officials confirmed that NYPD Chief of Department Jeffrey Maddrey suddenly resigned Friday evening, ahead of an explosive report alleging he repeatedly sexually assaulted a subordinate officer in exchange for overtime pay.

Maddrey was the department’s highest-ranking uniformed police officer. His accuser, Lieutenant Quathisha Epps, told the New York Post that he asked her to perform various sexual acts at NYPD headquarters.

“He wanted to have anal sex, vaginal sex, oral sex,” Epps told the Post. “He always asked me to kiss his penis.”

The Manhattan district attorney’s office said Saturday it had opened an investigation.

“These are extremely serious and disturbing allegations that allegedly occurred at NYPD headquarters in Manhattan,” said Doug Cohen, a spokesman for Manhattan District Attorney Alvin Bragg.

Maddrey’s departure follows a reorganization of the department under Police Commissioner Jessica Tisch, who took over the NYPD about a month ago. She made several personnel and policy changes, including ordering that 500 officers who had been “illegally transferred” return to their posts. On Thursday, she announced that she had replaced Tarik Sheppard, who was in charge of communications for the department. Sheppard clashed with reporters and former interim commissioner Tom Donlon.

In a statement, the mayor’s press secretary, Kayla Mamelak, said City Hall was “deeply disturbed” by the allegations against Maddrey, adding that “the NYPD is investigating this matter.”

“Mayor Adams is working closely with Police Commissioner Tisch as the NYPD conducts a separate department-wide review to ensure that no senior officers are using their power inappropriately,” Mamelak said. “We will not be making any further comment so as not to jeopardize either investigation.”

In a statement, the NYPD confirmed that Tisch had accepted Maddrey’s resignation Friday evening and would “thoroughly investigate” the matter.

On Saturday, Epps’ attorney, Eric Sanders, filed a discrimination charge with the Equal Employment Opportunity Commission, the first step before filing a federal lawsuit.

According to the complaint, Epps said that after she began rejecting Maddrey’s advances in October, he retaliated by orchestrating “an intentionally manipulated” overtime report that claimed she earned more than $200,000 in overtime pay.

After stories of her alleged overtime abuse came to light, Maddrey offered to speak to the commissioner on her behalf, but first asked her to kiss his penis, the complaint says. She said she “complied under duress.”

Immediately afterward, according to the complaint, she submitted her retirement paperwork and was investigated by the agency for her overtime work.

The NYPD also announced that John Chell, chief of patrol, would serve as interim department chief and Phil Rivera, chief of transportation, would serve as interim chief of patrol.

“The interim department and patrol chiefs will continue to lead efforts to reduce crime and disorder and build public trust,” Tisch said in a statement.

Maddrey was not mentioned in the press release and he could not be reached for comment.

Maddrey has been accused of sexual misconduct before. In 2016, he was sued by Tabatha Foster, a former police officer who said he demanded sex in his office. Both the federal and state lawsuits were ultimately dismissed.

NYPD Capt. Gabrielle Walls added Maddrey as a defendant in an ongoing sexual harassment lawsuit on Wednesday. Her complaint, filed in Manhattan Supreme Court, alleged that Maddrey repeatedly attempted to kiss her without her consent and also attempted to visit her in the district where she worked.

The complaint also alleges that Maddrey told Walls he would “mold” her and offered to help her with her career.

Maddrey also got into trouble for intervening in the arrest of a retired police officer who had been armed in pursuit of three boys. An independent oversight panel found he had abused his authority, but former Commissioner Edward Caban dismissed the charges in August.

Caban was forced to resign earlier this year after it was revealed that the U.S. Attorney’s Office for the Southern District and the Internal Revenue Service were investigating his alleged involvement in a shakedown plot with his twin brother.

Former Deputy Mayor for Public Safety Phil Banks, also a former police officer, was forced to resign amid a separate federal investigation.

Walls’ lawsuit is one of several lawsuits filed by female police officers that portray a culture of sexual harassment in the NYPD that has been repeatedly ignored by high-ranking police officials. Timothy Pearson, another former police officer and close confidant of Mayor Eric Adams, has been accused of sexually harassing several women at the NYPD. He also had to resign amid a separate federal investigation.

Adams, a former NYPD captain, is being sued by a former transit police colleague who accused him of sexual assault in 1993. The lawsuit was filed last year under the Adult Survivors Act.

The mayor has denied any wrongdoing.

This is a developing story and has been updated with more information.

Correction: An earlier version of this story misstated the date of a previous sexual abuse lawsuit against Maddrey.